Background.The immoderation of mitochondrial fission is one of the main contributors in ischemia reperfusion injury (IRI) and mesenchymal stromal cells (MSCs) derived extracellular vesicles have been regarded as a potential therapy method.Here, we hypothesized that extracellular vesicles (EVs) derived from human Wharton Jelly mesenchymal stromal cells (hWJMSCs) ameliorate Wall Decor acute renal IRI by inhibiting mitochondrial fission through miR-30b/c/d.Methods.EVs isolated from the condition medium of MCS were injected intravenously in rats immediately after monolateral nephrectomy and renal pedicle occlusion for 45 minutes.
Animals were sacrificed at 24 h after reperfusion and samples were collected.MitoTracker Red staining was used to see the morphology of the mitochondria.The expression of DRP1 was measured by western blot.miR-30 in EVs and rat tubular epithelial cells was assessed by qRT-PCR.Apoptosis pathway was identified by immunostaining.
Results.We found that the expression of miR-30 in injured kidney tissues was declined and mitochondrial dynamics turned to fission.But they were both restored in EVs group in parallel with reduced cell apoptosis.What is more, when the miR-30 antagomirs were used to reduce the miRNA levels, all the related effects of EVs reduced remarkably.Conclusion.
A single administration of hWJMSC-EVs could protect the kidney from IRI by Soccer - Shoes Turf - Junior inhibition of mitochondrial fission via miR-30.